Journal: Frontiers in Immunology

Article Title: Inflammatory landscape of Gardnerella vaginalis -associated bacterial vaginosis in rats: an integrated multi-omics analysis

doi: 10.3389/fimmu.2026.1914434

Figure Lengend Snippet: Upregulation of proteins associated with uterine TLR2/4–NF-κB–COX-2 inflammatory signaling in rats with Gardnerella vaginalis -associated bacterial vaginosis. (A) Relative protein abundance of Toll-like receptor 2 (TLR2), TLR4, phospho-NF-κB p65 at serine 536 (pS536-p65) and cyclooxygenase-2 (COX-2) in uterine tissues. Data are presented as the means ( X ¯ ) ± standard error of the mean (SEM); n = 3 independent uterine tissue samples per group. Statistical significance was determined using Student’s t-tests . Complete densitometric values are provided in Supplementary Table 11 . (B) Representative Western blot images. Protein levels were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Original uncropped Western blot images are provided in Supplementary Figure 1 .

Article Snippet: After blocking with a rapid blocking buffer (Epizyme, Shanghai, China), the blots were incubated overnight at 4 °C with the following primary antibodies: TLR2 (Abcam, Cambridge, UK; dilution 1:1000), TLR4 (Proteintech, Hubei, China; dilution 1:1000), phospho-NF-κB p65 at serine 536 (pS536-p65; Proteintech; dilution 1:2000), cyclooxygenase-2 (COX-2; Proteintech; dilution 1:1000), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; Proteintech; dilution 1:10000).

Techniques: Quantitative Proteomics, Tissue, Western Blot

Journal: Frontiers in Microbiology

Article Title: Qijiao Shengbai capsule ameliorates ulcerative colitis in mice by modulating gut microbiota and protecting the intestinal barrier, associated with suppression of the TLR4/MyD88/NF-κB pathway

doi: 10.3389/fmicb.2026.1875650

Figure Lengend Snippet: QJSB alleviates inflammation in DSS-induced UC mice in vivo ( n = 6). (A) , (B) , (C) and (D) Levels of MPO, IL-6, IL-1β, and TNF-α in the colon; (E) , (F) and (G) serum levels of IL-1β, IL-6, and TNF-α; (H) levels of LPS in feces. (I) protein blots for iNOS and COX-2; (J) Relative expression levels of iNOS; (K) relative expression levels of COX-2; (L) correlation analysis between inflammatory mediators and colon-specific metabolites. (### p < 0.001, ## p < 0.01, # p vs. Control; * p < 0.05, ** p < 0.01, *** p < 0.001vs. Model).

Article Snippet: TLR4, MyD88, IκBα, p-IκBα, NF-κB p65, NF-κB p-p65, iNOS, and COX-2 antibodies were obtained from Proteintech (Wuhan, China).

Techniques: In Vivo, Expressing, Analysis, Control

Journal: Nature cancer

Article Title: Copper depletion inhibits electron transport chain activity and nucleotide synthesis to boost leukemia therapy

doi: 10.1038/s43018-026-01177-4

Figure Lengend Snippet: (a) Western blot for COX2, COX1 and COX4 in BCS-treated SEM (day 8), NALM6 (day 6) and MOLT16 (day 8) cells. (b) Blue-native (BN-PAGE) followed by western blot detection of indicated subunits in native complex I-III in BCS treated SEM cells (day 8) or NALM6 cells (day 6). Representative data of two biological replicates shown. Additional replicate shown in Source Data. (c-d) Levels of NAD, NADH and the NAD/NADH ratio were determined by LC-MS targeted metabolite profiling in (c) SLC31A1-KO SEM cells or (d) BCS treated SEM (day 8), NALM6 (day 6) and MOLT16 (day 8) cells. (e) Levels of succinate, fumarate, and succinate/fumarate ratio in BCS treated SEM and NALM6 cells as determined by targeted LC-MS metabolite profiling. (f-g) Seahorse assay to measure OCR in permeabilized BCS-treated (f) SEM (day 8) or (g) NALM6 (day 6) cells following treatment with complex I or II substrates. (h-i) MitoStress Seahorse assay performed on SLC31A1-KO SEM cells with (h) eGFP or SLC31A1 rescue, or (i) 100 μM CuCl 2 pre-treatment for 24 hours. For Seahorse assays, plotted data are mean ± SD with 4–6 technical replicates (wells). Other plotted data are mean±SD, p-values from Šídák's multiple comparisons test. Each dot represents a biological replicate. BCS and CuCl 2 were used at 50 μM. OE = overexpression, BCS = bathocuproinedisulfonic acid, oligo = oligomycin, AA = antimycin A, rot = rotenone, FCCP = carbonyl cyanide-p-trifluoromethoxyphenylhydrazone, perm = XF Plasma Membrane Permeabilizer, pyr/mal = pyruvate and malate, succ = succinate.

Article Snippet: Immunoblotting was performed using the following primary antibodies: For reducing and denaturing western blot: vinculin (CST 13901; 1:2500) Beta actin (CST 8457; 1:2000) GAPDH (CST 2118L; 1:2000) MT-CO1/COX1 (abcam ab14705; 1:2000) COX4I1/COX4 (CST 4850; 1:1000) COX2 (Proteintech 55070–1-AP; 1:5000) DHODH (Santa Cruz E-8 sc-166348; 1:500) SDHA (CST 5839S; 1:1000) SLC31A1 (CST 13086S; 1:500) Beta tubulin (CST 2128P; 1:1000) Human Total OxPhos Cocktail (abcam ab110411; 1:1000) VDAC1/porin (abcam ab154856; 1:1000) Total ERK1/2 (CST 4696S; 1:1000) Phospho-ERK1/2 (CST 4370L; 1:1000) Primary antibodies were diluted in Signal Enhancer HIKARI Solution 1 (Nacalai USA NU00102).

Techniques: Activity Assay, Western Blot, Liquid Chromatography with Mass Spectroscopy, Profiling, Over Expression, Clinical Proteomics, Membrane